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96 well imaging plates  (Greiner Bio)


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    Greiner Bio 96 well imaging plates
    96 Well Imaging Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 95/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/96+well+imaging+plates/Screenstar+Microplate+96+Well+Coc+F-Bottom/bio_rxiv__64898__2026__03__18__712671-235-18-23
    Average 95 stars, based on 65 article reviews
    96 well imaging plates - by Bioz Stars, 2026-10
    95/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Photoporation enables non-viral delivery of prime editing RNP complexes into human iPSC-derived cardiomyocytes for cardiac genome correction.
    Article Snippet: .. Cells were seeded in black 96-well imaging plates (#655090, Greiner CellStar) at 100,000 cells/well and incubated for 24 h. After SpGPEmax RNP delivery, cells were cultured for another 24 h and stained with Hoechst 33342 (2 μg/mL) for nuclear visualization. .. Imaging was performed using a CV7000 confocal microscope (Yokogawa Corporation, Japan), with FITC (green) and Cy5 (red) fluorescence channels used to monitor RNP localization.

    Article Title: Ca V 1.2-dependent excitation-transcription coupling modulates nociception
    Article Snippet: We applied the ViewRNA Cell Plus Assay (Thermo Fisher Scientific, #88-19000) to combine the immunocytochemical detection of the neuronal marker UCHL1 and fluorescent in situ hybridization against respective target transcripts Bdnf , Fos , Npas4 , Inhba , and Rasd1 . .. In brief, mouse DRG cultures were prepared in poly-L-ornithine (0.1 mg/ml)/laminin (5 μg/ml)-precoated 96-well imaging plates (Greiner, #655090) and incubated overnight (37 °C, 5% CO 2 ). ..

    Article Title: The CRAC channel inhibitor BTP2 restricts Tulane virus and human norovirus replication independent of store-operated calcium entry
    Article Snippet: Thapsigargin was purchased from Thermo Scientific (CAS 67526-95-8) and used at a final concentration of 1 μM. .. MA104G6s cells were seeded on μClear 96-well imaging plates (Greiner) at a density of 50,000 cells per well in 10% FBS DMEM maintenance medium. ..

    Article Title: Dynamin independent endocytosis is an alternative cell entry mechanism for multiple animal viruses
    Article Snippet: .. MEF DKO cells were seeded into 25 cm 2 flask in growth medium containing 2 μM 4OH-tamoxifen (Sigma, cat. no. H-6278) for 48 h. Medium was replaced by growth medium containing 300 nM 4OH-tamoxifen and after two days, cells were trypsinized and seeded into 96-well imaging plates (Greiner Bio-One, cat. no. 655090). .. Cells were further incubated in medium containing 300 nM 4OH-tamoxifen for 48 h and then incubated with two-fold dilutions of AdV-C5-EGFP stock virus in DMEM medium (Sigma-Aldrich, cat. no. D6429) supplemented with 7.5% fetal calf serum (Gibco/ Thermo Fisher Scientific, cat. no. 10270106), 1% non-essential amino acids (Sigma-Aldrich, cat. no. M7145) and 1% penicillin-streptomycin (Sigma-Aldrich, cat. no. P0781).

    Article Title: Green and Chemical Synthesis of Iron Oxide Nanoparticles: Comparative Study for antimicrobial activity and Toxicity Concerns
    Article Snippet: Infections caused by multidrug-resistant bacteria in healthcare settings highlight the critical need for robust infection control strategies and effective antimicrobial stewardship.. This was further emphasized by the COVID19 pandemic.. This necessitates developing more effective antimicrobial agents.

    Article Title: Ca V 1.2-dependent excitation-transcription coupling modulates nociception
    Article Snippet: .. Viable cells were resuspended in Neurobasal A/B27 medium, plated in poly-L-ornithine (0.1 mg/ml)/laminin (5 μg/ml)-precoated 96-well imaging plates (Greiner, #655090) and incubated overnight (37°C, 5% CO 2 ). ..

    Article Title: Tumor Cell Death Drives Tumor-Promoting IL-6⁺ iCAF formation via P2X7-activation
    Article Snippet: CD3+ T cells were isolated from splenocyte of OT-I transgenic mouse (003831 - OT-1 Strain Details ( jax.org )) using the CD8-MACS (Miltenyi Biotec, 130-117-044) according to the manufacturer’s protocol. .. Target tumor cells (MC38-OVA cell line) were seeded 20 hours before the start of the experiment on fibronectin-coated 96-well imaging plates (Screenstar Microplate, Greiner Bio-One 655866; fibronectin: R&D Systems 1030-FN-05M). .. On the day of co-culture tumor cells were stained with CellTrackerTM Green CMFDA (Invitrogen, Thermo Fisher, C7025).

    Article Title: miR-23b neutralization in brain endothelium promotes blood-brain barrier repair through Wnt/β-catenin dependent and independent mechanisms
    Article Snippet: .. HBMECs in the OrganoPlate or on 96-well imaging plates (#655090, Greiner, Kremsmünster, Austria) were fixed with 4% paraformaldehyde in PBS or 100% methanol (-20°C), and permeabilized with 0.2% Triton X-100. ..

    Incubation:

    Article Title: Photoporation enables non-viral delivery of prime editing RNP complexes into human iPSC-derived cardiomyocytes for cardiac genome correction.
    Article Snippet: .. Cells were seeded in black 96-well imaging plates (#655090, Greiner CellStar) at 100,000 cells/well and incubated for 24 h. After SpGPEmax RNP delivery, cells were cultured for another 24 h and stained with Hoechst 33342 (2 μg/mL) for nuclear visualization. .. Imaging was performed using a CV7000 confocal microscope (Yokogawa Corporation, Japan), with FITC (green) and Cy5 (red) fluorescence channels used to monitor RNP localization.

    Article Title: Ca V 1.2-dependent excitation-transcription coupling modulates nociception
    Article Snippet: We applied the ViewRNA Cell Plus Assay (Thermo Fisher Scientific, #88-19000) to combine the immunocytochemical detection of the neuronal marker UCHL1 and fluorescent in situ hybridization against respective target transcripts Bdnf , Fos , Npas4 , Inhba , and Rasd1 . .. In brief, mouse DRG cultures were prepared in poly-L-ornithine (0.1 mg/ml)/laminin (5 μg/ml)-precoated 96-well imaging plates (Greiner, #655090) and incubated overnight (37 °C, 5% CO 2 ). ..

    Article Title: Ca V 1.2-dependent excitation-transcription coupling modulates nociception
    Article Snippet: .. Viable cells were resuspended in Neurobasal A/B27 medium, plated in poly-L-ornithine (0.1 mg/ml)/laminin (5 μg/ml)-precoated 96-well imaging plates (Greiner, #655090) and incubated overnight (37°C, 5% CO 2 ). ..

    Cell Culture:

    Article Title: Photoporation enables non-viral delivery of prime editing RNP complexes into human iPSC-derived cardiomyocytes for cardiac genome correction.
    Article Snippet: .. Cells were seeded in black 96-well imaging plates (#655090, Greiner CellStar) at 100,000 cells/well and incubated for 24 h. After SpGPEmax RNP delivery, cells were cultured for another 24 h and stained with Hoechst 33342 (2 μg/mL) for nuclear visualization. .. Imaging was performed using a CV7000 confocal microscope (Yokogawa Corporation, Japan), with FITC (green) and Cy5 (red) fluorescence channels used to monitor RNP localization.

    Staining:

    Article Title: Photoporation enables non-viral delivery of prime editing RNP complexes into human iPSC-derived cardiomyocytes for cardiac genome correction.
    Article Snippet: .. Cells were seeded in black 96-well imaging plates (#655090, Greiner CellStar) at 100,000 cells/well and incubated for 24 h. After SpGPEmax RNP delivery, cells were cultured for another 24 h and stained with Hoechst 33342 (2 μg/mL) for nuclear visualization. .. Imaging was performed using a CV7000 confocal microscope (Yokogawa Corporation, Japan), with FITC (green) and Cy5 (red) fluorescence channels used to monitor RNP localization.



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    YIPF5 regulates cellular and neuronal migration in vitro and in vivo (A) MCF10A WT, YIPF5-KO, and YIPF5-KO cells re-expressing either the I98S YIPF5 mutant or WT YIPF5 at low (blue) or high (violet) levels were plated in Incucyte <t>Imagelock</t> <t>96-well</t> plates and a wound healing assay performed using the Incucyte Wound Maker 96-Tool on the IncuCyte system. Displayed is the relative wound density at different time points as mean values ± SEM from three biological replicates. Statistical significance was evaluated using a two-way ANOVA with Tukey’s multiple comparisons test, with ∗∗ denoting p < 0.01 and ∗ indicating p < 0.05. (B) Schematic representation illustrating the in utero electroporation procedure at embryonic day 13.5 (E13.5) with a GFP-labeled plasmid encoding either shLuciferase or shYIPF5, followed by brain removal at E15.5. (C) Scheme of a coronal section of a mouse embryonic brain schematizing the position of the electrodes, the ventricles and the developing neocortex with the classification into distinct zones, ventricular zone (VZ), subventricular zone (SVZ), intermediate zone (IZ), and cortical plate (CP). This classification traces the cortical migration pattern of GFP + electroporated cells. Illustrations created with BioRender.com using an IHC image deriving from our murine brain. (D) Murine brains electroporated at E13.5 with plasmids expressing control shLuciferase or shYIPF5 were harvested at E15.5, fixed, sectioned with a vibratome, and immunostained for GFP to visualize transfected cells. Nuclear staining with Hoechst (not shown) facilitated the differentiation of brain regions, including VZ, SVZ, IZ, and CP. Representative images are shown. (E) Quantification of the proportion of EGFP + cells within specified cortical regions. Displayed are the means ± SEM (shLuciferase, n = 5 brains, 225 EGFP + cells; shYIPF5, n = 5 brains, 257 EGFP + cells). Statistical analysis: multiple unpaired t test. (F) Morphometric analysis of CP-localized neurons of shLuciferase and shYIPF5 electroporated brains. Images are maximum projections of sequential z sections, the inverted EGFP signal is depicted in black. (G) Quantification of neuron morphologies from shLuciferase ( n = 6 brains, 6 sections, 93 EGFP + cells) and shYIPF5 ( n = 5 brains, 6 sections, 71 cells) groups. Neurons were categorized into two groups: unbranched uni/bipolar and exhibiting complex arborizations. Mean values with standard deviation are shown, statistical analysis: one-way ANOVA with Šídák’s multiple comparisons test, ∗ p < 0.05. (H) Leading process orientation analysis of CP-localized neurons from brain sections as shown in (F). Arrowheads indicate examples of tilted neurites. The angles between the leading neurites of EGFP + neurons and the CP surface were calculated using the measurement feature in ImageJ. The frequency of angle values was categorized into specified ranges, expressed as percentages of the total number of values. The sample sizes comprised 92 EGFP + cells from six sections from six brains for the shLuciferase group and 96 EGFP + cells from nine sections from five brains for the shYIPF5 group. Displayed is the mean ± SEM. Independent unpaired t tests were conducted for the three angle ranges, with a significance level set at ∗ p < 0.05.
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    Image Search Results


    YIPF5 regulates cellular and neuronal migration in vitro and in vivo (A) MCF10A WT, YIPF5-KO, and YIPF5-KO cells re-expressing either the I98S YIPF5 mutant or WT YIPF5 at low (blue) or high (violet) levels were plated in Incucyte Imagelock 96-well plates and a wound healing assay performed using the Incucyte Wound Maker 96-Tool on the IncuCyte system. Displayed is the relative wound density at different time points as mean values ± SEM from three biological replicates. Statistical significance was evaluated using a two-way ANOVA with Tukey’s multiple comparisons test, with ∗∗ denoting p < 0.01 and ∗ indicating p < 0.05. (B) Schematic representation illustrating the in utero electroporation procedure at embryonic day 13.5 (E13.5) with a GFP-labeled plasmid encoding either shLuciferase or shYIPF5, followed by brain removal at E15.5. (C) Scheme of a coronal section of a mouse embryonic brain schematizing the position of the electrodes, the ventricles and the developing neocortex with the classification into distinct zones, ventricular zone (VZ), subventricular zone (SVZ), intermediate zone (IZ), and cortical plate (CP). This classification traces the cortical migration pattern of GFP + electroporated cells. Illustrations created with BioRender.com using an IHC image deriving from our murine brain. (D) Murine brains electroporated at E13.5 with plasmids expressing control shLuciferase or shYIPF5 were harvested at E15.5, fixed, sectioned with a vibratome, and immunostained for GFP to visualize transfected cells. Nuclear staining with Hoechst (not shown) facilitated the differentiation of brain regions, including VZ, SVZ, IZ, and CP. Representative images are shown. (E) Quantification of the proportion of EGFP + cells within specified cortical regions. Displayed are the means ± SEM (shLuciferase, n = 5 brains, 225 EGFP + cells; shYIPF5, n = 5 brains, 257 EGFP + cells). Statistical analysis: multiple unpaired t test. (F) Morphometric analysis of CP-localized neurons of shLuciferase and shYIPF5 electroporated brains. Images are maximum projections of sequential z sections, the inverted EGFP signal is depicted in black. (G) Quantification of neuron morphologies from shLuciferase ( n = 6 brains, 6 sections, 93 EGFP + cells) and shYIPF5 ( n = 5 brains, 6 sections, 71 cells) groups. Neurons were categorized into two groups: unbranched uni/bipolar and exhibiting complex arborizations. Mean values with standard deviation are shown, statistical analysis: one-way ANOVA with Šídák’s multiple comparisons test, ∗ p < 0.05. (H) Leading process orientation analysis of CP-localized neurons from brain sections as shown in (F). Arrowheads indicate examples of tilted neurites. The angles between the leading neurites of EGFP + neurons and the CP surface were calculated using the measurement feature in ImageJ. The frequency of angle values was categorized into specified ranges, expressed as percentages of the total number of values. The sample sizes comprised 92 EGFP + cells from six sections from six brains for the shLuciferase group and 96 EGFP + cells from nine sections from five brains for the shYIPF5 group. Displayed is the mean ± SEM. Independent unpaired t tests were conducted for the three angle ranges, with a significance level set at ∗ p < 0.05.

    Journal: iScience

    Article Title: The microcephaly-associated protein YIPF5 differentially regulates ER export

    doi: 10.1016/j.isci.2026.114791

    Figure Lengend Snippet: YIPF5 regulates cellular and neuronal migration in vitro and in vivo (A) MCF10A WT, YIPF5-KO, and YIPF5-KO cells re-expressing either the I98S YIPF5 mutant or WT YIPF5 at low (blue) or high (violet) levels were plated in Incucyte Imagelock 96-well plates and a wound healing assay performed using the Incucyte Wound Maker 96-Tool on the IncuCyte system. Displayed is the relative wound density at different time points as mean values ± SEM from three biological replicates. Statistical significance was evaluated using a two-way ANOVA with Tukey’s multiple comparisons test, with ∗∗ denoting p < 0.01 and ∗ indicating p < 0.05. (B) Schematic representation illustrating the in utero electroporation procedure at embryonic day 13.5 (E13.5) with a GFP-labeled plasmid encoding either shLuciferase or shYIPF5, followed by brain removal at E15.5. (C) Scheme of a coronal section of a mouse embryonic brain schematizing the position of the electrodes, the ventricles and the developing neocortex with the classification into distinct zones, ventricular zone (VZ), subventricular zone (SVZ), intermediate zone (IZ), and cortical plate (CP). This classification traces the cortical migration pattern of GFP + electroporated cells. Illustrations created with BioRender.com using an IHC image deriving from our murine brain. (D) Murine brains electroporated at E13.5 with plasmids expressing control shLuciferase or shYIPF5 were harvested at E15.5, fixed, sectioned with a vibratome, and immunostained for GFP to visualize transfected cells. Nuclear staining with Hoechst (not shown) facilitated the differentiation of brain regions, including VZ, SVZ, IZ, and CP. Representative images are shown. (E) Quantification of the proportion of EGFP + cells within specified cortical regions. Displayed are the means ± SEM (shLuciferase, n = 5 brains, 225 EGFP + cells; shYIPF5, n = 5 brains, 257 EGFP + cells). Statistical analysis: multiple unpaired t test. (F) Morphometric analysis of CP-localized neurons of shLuciferase and shYIPF5 electroporated brains. Images are maximum projections of sequential z sections, the inverted EGFP signal is depicted in black. (G) Quantification of neuron morphologies from shLuciferase ( n = 6 brains, 6 sections, 93 EGFP + cells) and shYIPF5 ( n = 5 brains, 6 sections, 71 cells) groups. Neurons were categorized into two groups: unbranched uni/bipolar and exhibiting complex arborizations. Mean values with standard deviation are shown, statistical analysis: one-way ANOVA with Šídák’s multiple comparisons test, ∗ p < 0.05. (H) Leading process orientation analysis of CP-localized neurons from brain sections as shown in (F). Arrowheads indicate examples of tilted neurites. The angles between the leading neurites of EGFP + neurons and the CP surface were calculated using the measurement feature in ImageJ. The frequency of angle values was categorized into specified ranges, expressed as percentages of the total number of values. The sample sizes comprised 92 EGFP + cells from six sections from six brains for the shLuciferase group and 96 EGFP + cells from nine sections from five brains for the shYIPF5 group. Displayed is the mean ± SEM. Independent unpaired t tests were conducted for the three angle ranges, with a significance level set at ∗ p < 0.05.

    Article Snippet: For live imaging, cells were cultured in a 96-Well Cell Imaging Plate with a cover glass bottom (Eppendorf, Cat. No. 0030 741.030).

    Techniques: Migration, In Vitro, In Vivo, Expressing, Mutagenesis, Wound Healing Assay, In Utero, Electroporation, Labeling, Plasmid Preparation, Control, Transfection, Staining, Standard Deviation